Hotspot

rps16

LSC · 4797-5941 · 1145 bp

Back to Origanum

Pi 0.000582
MarkerSeek score 33.40
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1145
variable_sites
1
pi
0.000582
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
33.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 ATATACAAGCCGCTACCCCC CCCGTTCCAAATTAGAGGAATG 55.0 45.5 59.0 57.1 1201 bp 1.0 48.7
rps16_p2 ATATACAAGCCGCTACCCCC CCCGTTCCAAATTAGAGGAA 55.0 45.0 59.0 55.0 1201 bp 1.0 48.3
rps16_p3 ATATACAAGCCGCTACCCCC CCCGTTCCAAATTAGAGGAAT 55.0 42.9 59.0 55.6 1201 bp 1.0 45.0
rps16_p4 TATACAAGCCGCTACCCCC CCCGTTCCAAATTAGAGGAATG 57.9 45.5 58.6 57.1 1200 bp 1.0 44.4
rps16_p5 TATACAAGCCGCTACCCCC CCCGTTCCAAATTAGAGGAA 57.9 45.0 58.6 55.0 1200 bp 1.0 44.0