Hotspot

rpoA

LSC · 76928-77935 · 1008 bp

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Pi 0.00132

MarkerSeek score 59.60

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1008
variable_sites: 2
pi: 0.00132
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	TGAATTGTGCTACAGATTCA	AATGGCTGTAGACCTCCCAA	35.0	50.0	52.5	58.3	1179 bp	1.0	40.1
rpoA_p2	TGAATTGTGCTACAGATTCA	ATGGCTGTAGACCTCCCAAA	35.0	50.0	52.5	58.3	1178 bp	1.0	40.1
rpoA_p3	TGAATTGTGCTACAGATTCAA	AATGGCTGTAGACCTCCCAA	33.3	50.0	53.4	58.3	1179 bp	1.0	40.1
rpoA_p4	TGAATTGTGCTACAGATTCAA	ATGGCTGTAGACCTCCCAAA	33.3	50.0	53.4	58.3	1178 bp	1.0	40.1
rpoA_p5	TGAATTGTGCTACAGATTCAAATCT	ATGGCTGTAGACCTCCCAAA	32.0	50.0	57.3	58.3	1178 bp	1.0	40.1