Hotspot

clpP1

LSC · 69195-71097 · 1903 bp

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Pi 0.0014

MarkerSeek score 58.80

Primer available yes

Label clpP1

Feature Metadata

label_name: clpP1
feature_type: gene
region: LSC
length_bp: 1903
variable_sites: 4
pi: 0.0014
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1_p1	TGGGCTGGTTTAGATTGATCC	ACGTTTCCACATCAAAGTGA	47.6	40.0	57.7	55.5	2062-2072 bp	1.0	49.9
clpP1_p2	TGGGCTGGTTTAGATTGATCC	ACGTTTCCACATCAAAGTGAA	47.6	38.1	57.7	56.3	2062-2072 bp	1.0	48.2
clpP1_p3	GGGCTGGTTTAGATTGATCCT	ACGTTTCCACATCAAAGTGA	47.6	40.0	57.4	55.5	2061-2071 bp	1.0	47.6
clpP1_p4	TGGGCTGGTTTAGATTGATCC	ACGTTTCCACATCAAAGTGAAA	47.6	36.4	57.7	57.0	2062-2072 bp	1.0	46.0
clpP1_p5	GGGCTGGTTTAGATTGATCCT	ACGTTTCCACATCAAAGTGAA	47.6	38.1	57.4	56.3	2061-2071 bp	1.0	45.9