Hotspot

trnL-UAG-ccsA

SSC · 112224-112325 · 102 bp

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Pi 0.0131

MarkerSeek score 60.80

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 102
variable_sites: 2
pi: 0.0131
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	TAGACACGCTGCTCTTAGGA	AGAATTGAAACAGTCGAAAAAGA	50.0	30.4	57.9	55.0	225 bp	1.0	40.7
trnL-UAG-ccsA_p2	AAATTGGTAGACACGCTGCT	AGAATTGAAACAGTCGAAAAAGA	45.0	30.4	57.8	55.0	232 bp	1.0	40.7
trnL-UAG-ccsA_p3	GGTAGACACGCTGCTCTTAG	AGAATTGAAACAGTCGAAAAAGA	55.0	30.4	57.8	55.0	227 bp	1.0	40.7
trnL-UAG-ccsA_p4	GTAGACACGCTGCTCTTAGG	AGAATTGAAACAGTCGAAAAAGA	55.0	30.4	57.8	55.0	226 bp	1.0	40.7
trnL-UAG-ccsA_p5	ACAAGCCGCTATGGTGAAAT	AGAATTGAAACAGTCGAAAAAGA	45.0	30.4	57.9	55.0	248 bp	1.0	40.6