Hotspot

rpl16-rps3

LSC · 80582-81595 · 1014 bp

Back to Origanum

Pi 0.00197

MarkerSeek score 60.00

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 1014
variable_sites: 3
pi: 0.00197
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	GTTCAAGTGCCTGAAGAGCA	ATCTATGGGGTCTTGGGCAT	50.0	50.0	58.4	58.2	1189-1190 bp	1.0	75.7
rpl16-rps3_p2	GGGTTCAAGTGCCTGAAGAG	ATCTATGGGGTCTTGGGCAT	55.0	50.0	58.5	58.2	1191-1192 bp	1.0	75.1
rpl16-rps3_p3	TTCAAGTGCCTGAAGAGCAT	ATCTATGGGGTCTTGGGCAT	45.0	50.0	57.4	58.2	1188-1189 bp	1.0	74.3
rpl16-rps3_p4	GTTCAAGTGCCTGAAGAGCA	CCCTACAAACCATTCGAGCT	50.0	50.0	58.4	57.6	1242-1243 bp	1.0	73.7
rpl16-rps3_p5	GTTCAAGTGCCTGAAGAGCA	GGGGTCTTGGGCATCAAAAT	50.0	50.0	58.4	58.4	1183-1184 bp	1.0	73.6