Hotspot

rpl20-rps12

LSC · 68149-68956 · 808 bp

Back to Origanum

Pi 0.00165
MarkerSeek score 59.80
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
808
variable_sites
2
pi
0.00165
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 TCCGAGCTATATATCCGCGT ACAGCCCATTCGAAATGTCA 50.0 45.0 58.0 57.8 921-922 bp 1.0 78.3
rpl20-rps12_p2 TCCGAGCTATATATCCGCGT ACAAGACAGCCCATTCGAAA 50.0 45.0 58.0 57.7 926-927 bp 1.0 77.7
rpl20-rps12_p3 ACCCTCGAAAACTTGATGCA ACAGCCCATTCGAAATGTCA 45.0 45.0 57.7 57.8 966-967 bp 1.0 76.4
rpl20-rps12_p4 TCCGAGCTATATATCCGCGT AGACAGCCCATTCGAAATGT 50.0 45.0 58.0 57.5 923-924 bp 1.0 75.9
rpl20-rps12_p5 ACCCTCGAAAACTTGATGCA ACAAGACAGCCCATTCGAAA 45.0 45.0 57.7 57.7 971-972 bp 1.0 75.8