Hotspot

psaA-pafI

LSC · 41211-41960 · 750 bp

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Pi 0

MarkerSeek score 60.10

Primer available yes

Label psaA-pafI

Feature Metadata

label_name: psaA-pafI
feature_type: igs
region: LSC
length_bp: 750
variable_sites: 0
pi: 0
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-pafI_p1	GCCCATTCCTCGAAAGAAGT	ATTGGTTGAAGATCACGGGG	50.0	50.0	57.9	57.9	853-862 bp	1.0	78.9
psaA-pafI_p2	GAAAAATGACCCGGTTTGGC	ATTGGTTGAAGATCACGGGG	50.0	50.0	58.2	57.9	871-880 bp	1.0	78.1
psaA-pafI_p3	GCCCATTCCTCGAAAGAAGT	TGGTTTGATCAAGCTGCTGA	50.0	45.0	57.9	57.7	926-935 bp	1.0	77.6
psaA-pafI_p4	GAAAAATGACCCGGTTTGGC	TGGTTTGATCAAGCTGCTGA	50.0	45.0	58.2	57.7	944-953 bp	1.0	76.9
psaA-pafI_p5	GCCCATTCCTCGAAAGAAGT	CGGAGGCTTGGTTTGATCAA	50.0	50.0	57.9	58.5	934-943 bp	1.0	76.1