Hotspot

trnS-UGA-psbZ

LSC · 35053-35386 · 334 bp

Back to Origanum

Pi 0.002

MarkerSeek score 40.50

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 334
variable_sites: 1
pi: 0.002
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAACCACTCAGCCATCTCTC	CGCAAAAACAGCCAATTGGA	55.0	45.0	58.0	58.1	388-393 bp	1.0	78.9
trnS-UGA-psbZ_p2	CAACCACTCAGCCATCTCTC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.0	57.8	454-459 bp	1.0	78.4
trnS-UGA-psbZ_p3	GTTTTCAAGACCGGGGCTAT	CGCAAAAACAGCCAATTGGA	50.0	45.0	57.9	58.1	408-413 bp	1.0	78.1
trnS-UGA-psbZ_p4	CAACCACTCAGCCATCTCTC	GACCAACCATCAGGAGAAGC	55.0	55.0	58.0	58.3	456-461 bp	1.0	78.0
trnS-UGA-psbZ_p5	GTTTTCAAGACCGGGGCTAT	CCAACCATCAGGAGAAGCAA	50.0	50.0	57.9	57.8	474-479 bp	1.0	77.5