Hotspot

trnT-GGU-psbD

LSC · 30937-32282 · 1346 bp

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Pi 0.00149

MarkerSeek score 59.90

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1346
variable_sites: 3
pi: 0.00149
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	ACAGAGTGACTCGTTCAGGA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.0	58.1	1552-1554 bp	1.0	79.2
trnT-GGU-psbD_p2	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	55.0	50.0	58.1	58.1	1504-1506 bp	1.0	78.8
trnT-GGU-psbD_p3	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1507-1509 bp	1.0	78.7
trnT-GGU-psbD_p4	ACAGAGTGACTCGTTCAGGA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.0	58.2	1542-1544 bp	1.0	78.2
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1494-1496 bp	1.0	77.8