Hotspot

rps18

LSC · 68206-68511 · 306 bp

Back to Oreosalsola

Pi 0.0163

MarkerSeek score 47.20

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: LSC
length_bp: 306
variable_sites: 5
pi: 0.0163
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	CTAGTCGGTTGGAATTGCGA	CGTTTGTGGATCACTCGGAT	50.0	50.0	58.0	58.0	1055-1061 bp	1.0	81.5
rps18_p2	CTAGTCGGTTGGAATTGCGA	TCGTCGTTTGTGGATCACTC	50.0	50.0	58.0	57.9	1059-1065 bp	1.0	81.1
rps18_p3	ATAGATCGAACGGGGTGTCT	CGTTTGTGGATCACTCGGAT	50.0	50.0	57.9	58.0	985-991 bp	1.0	81.1
rps18_p4	TTTTCCCAGATGCGTTGACA	CGTTTGTGGATCACTCGGAT	45.0	50.0	58.0	58.0	1480-1487 bp	1.0	81.0
rps18_p5	TTTTCCCAGATGCGTTGACA	TCGTCGTTTGTGGATCACTC	45.0	50.0	58.0	57.9	1484-1491 bp	1.0	80.6