Hotspot

ndhI

SSC · 118622-119134 · 513 bp

Back to Oreosalsola

Pi 0.0156

MarkerSeek score 52.00

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 513
variable_sites: 8
pi: 0.0156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	GAAGTAATCCCGTCCCCAAC	GCGGCAACCCATAGGATTTA	55.0	50.0	58.0	58.0	1694-1696 bp	1.0	80.3
ndhI_p2	GAAGTAATCCCGTCCCCAAC	CAGAATGCGAGAGATTGCCT	55.0	50.0	58.0	58.0	1650-1652 bp	1.0	80.2
ndhI_p3	GCGCAGAAGCTACAAAATGG	GCGGCAACCCATAGGATTTA	50.0	50.0	58.1	58.0	1576-1578 bp	1.0	79.9
ndhI_p4	ATGAAGTAATCCCGTCCCCA	GCGGCAACCCATAGGATTTA	50.0	50.0	58.1	58.0	1696-1698 bp	1.0	79.7
ndhI_p5	GCGCAGAAGCTACAAAATGG	CAGAATGCGAGAGATTGCCT	50.0	50.0	58.1	58.0	1532-1534 bp	1.0	79.7