Hotspot

atpF

LSC · 11495-12827 · 1333 bp

Back to Oreosalsola

Pi 0.00975

MarkerSeek score 50.60

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1333
variable_sites: 13
pi: 0.00975
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CACGCCCCAAATAAGACTCA	CCCCGGGAAGGACTTATTTG	50.0	55.0	57.9	57.9	2019-2020 bp	1.0	80.0
atpF_p2	ACACGCCCCAAATAAGACTC	CCCCGGGAAGGACTTATTTG	50.0	55.0	57.9	57.9	2020-2021 bp	1.0	80.0
atpF_p3	ACGCCCCAAATAAGACTCAC	CCCCGGGAAGGACTTATTTG	50.0	55.0	57.9	57.9	2018-2019 bp	1.0	80.0
atpF_p4	CACGCCCCAAATAAGACTCA	TCCCATGAAACCCAGTACCT	50.0	50.0	57.9	57.9	1909 bp	0.5	58.7
atpF_p5	ACGCCCCAAATAAGACTCAC	TCCCATGAAACCCAGTACCT	50.0	50.0	57.9	57.9	1908 bp	0.5	58.7