Hotspot

trnN-GUU-ndhF

IRb · 107712-109208 · 1497 bp

Back to Oreosalsola

Pi 0.0323

MarkerSeek score 78.60

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 1497
variable_sites: 47
pi: 0.0323
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	AGGATTCGAACCTACGACCA	GTATGGAGGAGGGAGTCGAA	50.0	55.0	58.2	57.9	1615-1650 bp	1.0	82.6
trnN-GUU-ndhF_p2	AGGATTCGAACCTACGACCA	AAGTATGGAGGAGGGAGTCG	50.0	55.0	58.2	57.9	1617-1652 bp	1.0	82.5
trnN-GUU-ndhF_p3	AATCAGTTAACAGCCGACCG	GTATGGAGGAGGGAGTCGAA	50.0	55.0	58.3	57.9	1596-1631 bp	1.0	81.6
trnN-GUU-ndhF_p4	AATCAGTTAACAGCCGACCG	AAGTATGGAGGAGGGAGTCG	50.0	55.0	58.3	57.9	1598-1633 bp	1.0	81.6
trnN-GUU-ndhF_p5	CCCCAAGTAGGATTCGAACC	GTATGGAGGAGGGAGTCGAA	55.0	55.0	57.7	57.9	1623-1658 bp	1.0	81.2