Hotspot

rps18-rpl20

LSC · 68512-68744 · 233 bp

Back to Oreosalsola

Pi 0.03

MarkerSeek score 69.80

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 233
variable_sites: 7
pi: 0.03
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	AAGAACCGAGTCGACTACGA	TCGTAAAATACTTGCCCAAATAGC	50.0	37.5	58.2	57.9	394 bp	1.0	46.8
rps18-rpl20_p2	AAGAACCGAGTCGACTACGA	CGTAAAATACTTGCCCAAATAGCT	50.0	37.5	58.2	57.9	393 bp	1.0	46.8
rps18-rpl20_p3	AAGAACCGAGTCGACTACGA	CGTAAAATACTTGCCCAAATAGC	50.0	39.1	58.2	56.6	393 bp	1.0	44.3
rps18-rpl20_p4	AAAGAACCGAGTCGACTACG	TCGTAAAATACTTGCCCAAATAGC	50.0	37.5	57.4	57.9	395 bp	1.0	43.5
rps18-rpl20_p5	AAAGAACCGAGTCGACTACG	CGTAAAATACTTGCCCAAATAGCT	50.0	37.5	57.4	57.9	394 bp	1.0	43.5