Hotspot

psbE-petL

LSC · 64840-66077 · 1238 bp

Back to Oreosalsola

Pi 0.0138

MarkerSeek score 65.00

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1238
variable_sites: 17
pi: 0.0138
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGCGGAGGTAATAGTTAAAGCC	50.0	45.5	57.9	57.9	1402-1418 bp	1.0	64.3
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GGAGGTAATAGTTAAAGCCGCT	50.0	45.5	57.9	57.9	1399-1415 bp	1.0	64.3
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	AGCGGAGGTAATAGTTAAAGCC	50.0	45.5	57.8	57.9	1325-1341 bp	1.0	63.5
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	GGAGGTAATAGTTAAAGCCGCT	50.0	45.5	57.8	57.9	1322-1338 bp	1.0	63.5
psbE-petL_p5	ACGAATAACCAACCCGCAAT	GGAGGTAATAGTTAAAGCCGCT	45.0	45.5	58.2	57.9	1408-1424 bp	1.0	63.3