Hotspot

psaI-ycf4

LSC · 59954-60304 · 351 bp

Back to Oreosalsola

Pi 0.0199

MarkerSeek score 65.00

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 351
variable_sites: 7
pi: 0.0199
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	ATTTCCGGCAATTGCAATGG	ACGTTCTGATCGCCAATTCA	45.0	45.0	58.0	57.9	433 bp	1.0	80.3
psaI-ycf4_p2	ATTTCCGGCAATTGCAATGG	GGATAAAGGCCCAGCAGAAA	45.0	50.0	58.0	57.9	494 bp	1.0	79.8
psaI-ycf4_p3	TAGGCCTAGTATTTCCGGCA	ACGTTCTGATCGCCAATTCA	50.0	45.0	57.9	57.9	443 bp	1.0	79.8
psaI-ycf4_p4	ATTTCCGGCAATTGCAATGG	AAAGGATAAAGGCCCAGCAG	45.0	50.0	58.0	57.9	497 bp	1.0	79.7
psaI-ycf4_p5	GGCAATTGCAATGGCTTCTT	ACGTTCTGATCGCCAATTCA	45.0	45.0	57.9	57.9	427 bp	1.0	79.7