Hotspot

atpB-rbcL

LSC · 54772-55536 · 765 bp

Back to Oreosalsola

Pi 0.0132

MarkerSeek score 67.20

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 765
variable_sites: 10
pi: 0.0132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GGAGGAAAAGCTACGTCCAG	AGTCTCTGTTTGTGGTGACA	55.0	45.0	58.0	56.6	892-896 bp	1.0	70.5
atpB-rbcL_p2	GTTTTCAAGTGCGGAAACCC	AGTCTCTGTTTGTGGTGACA	50.0	45.0	58.2	56.6	833-837 bp	1.0	69.4
atpB-rbcL_p3	AAAAGCTACGTCCAGTACCG	AGTCTCTGTTTGTGGTGACA	50.0	45.0	57.6	56.6	887-891 bp	1.0	67.7
atpB-rbcL_p4	CAGGACCCGAAGTAGTAGGA	AGTCTCTGTTTGTGGTGACA	55.0	45.0	57.6	56.6	813-817 bp	1.0	67.2
atpB-rbcL_p5	GGAGGAAAAGCTACGTCCAG	AGTCTCTGTTTGTGGTGACAT	55.0	42.9	58.0	57.2	892-896 bp	1.0	66.7