Hotspot

psaA-ycf3

LSC · 41770-42532 · 763 bp

Back to Oreosalsola

Pi 0.0144

MarkerSeek score 56.10

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 763
variable_sites: 11
pi: 0.0144
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TTTTACTTCTGGTTCCGGCG	ATTGGTTGAAGATCACGGGG	50.0	50.0	58.5	57.9	828-832 bp	1.0	76.2
psaA-ycf3_p2	CGAAGTTTTTACGGGATCCCT	ATTGGTTGAAGATCACGGGG	47.6	50.0	58.0	57.9	861-865 bp	1.0	71.8
psaA-ycf3_p3	ACGGGATCCCTATCTACCAA	ATTGGTTGAAGATCACGGGG	50.0	50.0	56.9	57.9	851-855 bp	1.0	71.3
psaA-ycf3_p4	TTTACTTCTGGTTCCGGCG	ATTGGTTGAAGATCACGGGG	52.6	50.0	57.8	57.9	827-831 bp	1.0	70.3
psaA-ycf3_p5	ACGAAGTTTTTACGGGATCCC	ATTGGTTGAAGATCACGGGG	47.6	50.0	58.3	57.9	862-866 bp	1.0	69.6