Hotspot

atpH-atpI

LSC · 13497-14206 · 710 bp

Back to Oreosalsola

Pi 0.0191

MarkerSeek score 58.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 710
variable_sites: 13
pi: 0.0191
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GCCAATGACCCAAGAAAACG	GGCAAATAGGGGGTTTCCAA	50.0	50.0	57.9	58.1	2062-2084 bp	1.0	81.4
atpH-atpI_p2	AGGTACTGGGTTTCATGGGA	GGCAAATAGGGGGTTTCCAA	50.0	50.0	57.9	58.1	1853-1873 bp	1.0	81.3
atpH-atpI_p3	AGGTACTGGGTTTCATGGGA	TTGGCAAATAGGGGGTTTCC	50.0	50.0	57.9	58.1	1855-1875 bp	1.0	81.3
atpH-atpI_p4	CAAATAAGTCCTTCCCGGGG	GGCAAATAGGGGGTTTCCAA	55.0	50.0	57.9	58.1	1747-1763 bp	1.0	81.2
atpH-atpI_p5	CAAATAAGTCCTTCCCGGGG	TTGGCAAATAGGGGGTTTCC	55.0	50.0	57.9	58.1	1749-1765 bp	1.0	81.2