Hotspot

atpF-atpH

LSC · 12828-13250 · 423 bp

Back to Oreosalsola

Pi 0.0146

MarkerSeek score 65.90

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 423
variable_sites: 6
pi: 0.0146
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GCCAATGACCCAAGAAAACG	GCAGAGGGAAAAATACGAGGT	50.0	47.6	57.9	58.0	574-580 bp	1.0	73.7
atpF-atpH_p2	TGGCCAATGACCCAAGAAAA	GCAGAGGGAAAAATACGAGGT	45.0	47.6	57.9	58.0	576-582 bp	1.0	73.5
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	GCAGAGGGAAAAATACGAGGT	50.0	47.6	57.9	58.0	600-606 bp	1.0	73.2
atpF-atpH_p4	GATGGCCAATGACCCAAGAA	GCAGAGGGAAAAATACGAGGT	50.0	47.6	58.1	58.0	578-584 bp	1.0	73.0
atpF-atpH_p5	GCCAATGACCCAAGAAAACG	AGCAGAGGGAAAAATACGAGG	50.0	47.6	57.9	57.7	575-581 bp	1.0	71.7