Hotspot

trnK-UUU-rps16

LSC · 4183-4904 · 722 bp

Back to Oreosalsola

Pi 0.025
MarkerSeek score 73.40
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
722
variable_sites
18
pi
0.025
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT TGCTCAACCTACCGAAACTG 50.0 50.0 58.2 57.8 812-814 bp 1.0 79.3
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT TGCTCAACCTACCGAAACTG 50.0 50.0 58.2 57.8 811-813 bp 1.0 79.3
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT GCTCAACCTACCGAAACTGT 50.0 50.0 58.2 57.8 811-813 bp 1.0 79.3
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT GCTCAACCTACCGAAACTGT 50.0 50.0 58.2 57.8 810-812 bp 1.0 79.3
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT GTGCTCAACCTACCGAAACT 50.0 50.0 58.2 57.8 812-814 bp 1.0 79.3