Hotspot

psbA-trnK-UUU

LSC · 1385-1609 · 225 bp

Back to Oreosalsola

Pi 0.014

MarkerSeek score 64.80

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 225
variable_sites: 3
pi: 0.014
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	ACGGTTTTCAGTGCTGGTTA	GAAAGCCGTGTGCAATGAAA	45.0	45.0	57.7	57.9	414-424 bp	1.0	78.7
psbA-trnK-UUU_p2	ACGGTTTTCAGTGCTGGTTA	CGGTTTGGGGAGAGGTTTTT	45.0	50.0	57.7	58.3	384-394 bp	1.0	77.6
psbA-trnK-UUU_p3	TACAGAAGCGACCCCATAGG	GAAAGCCGTGTGCAATGAAA	55.0	45.0	58.6	57.9	388-398 bp	1.0	76.9
psbA-trnK-UUU_p4	ACGGTTTTCAGTGCTGGTTA	AAAATGCAAGCACGGTTTGG	45.0	45.0	57.7	58.4	396-406 bp	1.0	76.6
psbA-trnK-UUU_p5	ATCCAGTTACAGAAGCGACC	GAAAGCCGTGTGCAATGAAA	50.0	45.0	57.3	57.9	395-405 bp	1.0	76.1