Hotspot

ycf1

SSC · 127219-132582 · 5364 bp

Back to Oreorchis

Pi 0.00895
MarkerSeek score 48.30
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5364
variable_sites
72
pi
0.00895
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TGGTATGATCCAAGGTTGAA TCCAGAGATCAAACCATGCA 40.0 45.0 53.9 56.8 178 bp 1.0 42.7
ycf1_p2 TTGGTATGATCCAAGGTTGA TCCAGAGATCAAACCATGCA 40.0 45.0 53.9 56.8 179 bp 1.0 42.7
ycf1_p3 TTGGTATGATCCAAGGTTGAA TCCAGAGATCAAACCATGCA 38.1 45.0 54.8 56.8 179 bp 1.0 42.7
ycf1_p4 TGGTATGATCCAAGGTTGAA TCCAGAGATCAAACCATGCAA 40.0 42.9 53.9 57.6 178 bp 1.0 42.7
ycf1_p5 TTGGTATGATCCAAGGTTGA TCCAGAGATCAAACCATGCAA 40.0 42.9 53.9 57.6 179 bp 1.0 42.7