Hotspot

ndhD

SSC · 118677-120185 · 1509 bp

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Pi 0.00619

MarkerSeek score 53.00

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1509
variable_sites: 14
pi: 0.00619
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	CGCGAGAATATGTGAAAATAGGA	ATTATTCTTCTCCCGAGCGC	39.1	50.0	56.8	57.8	1595-1658 bp	1.0	47.6
ndhD_p2	TCGCGAGAATATGTGAAAATAGG	ATTATTCTTCTCCCGAGCGC	39.1	50.0	56.8	57.8	1596-1659 bp	1.0	47.6
ndhD_p3	TCGCGAGAATATGTGAAAATAGGA	ATTATTCTTCTCCCGAGCGC	37.5	50.0	58.2	57.8	1596-1659 bp	1.0	47.6
ndhD_p4	CGCGAGAATATGTGAAAATAGGAA	ATTATTCTTCTCCCGAGCGC	37.5	50.0	57.5	57.8	1595-1658 bp	1.0	44.7
ndhD_p5	CGCGAGAATATGTGAAAATAGG	ATTATTCTTCTCCCGAGCGC	40.9	50.0	55.4	57.8	1595-1658 bp	1.0	44.4