Hotspot

psaC-ndhE

SSC · 120561-121144 · 584 bp

Back to Oreorchis

Pi 0.0228

MarkerSeek score 62.30

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 584
variable_sites: 20
pi: 0.0228
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	ACATCCGTCCCAAGGTATCA	ACTGATCACAAGCCAAAGCA	50.0	45.0	58.1	58.0	918-962 bp	1.0	82.2
psaC-ndhE_p2	ATTGGCCAAGCTGCTAAAGT	ACTGATCACAAGCCAAAGCA	45.0	45.0	58.1	58.0	1514-1558 bp	1.0	81.7
psaC-ndhE_p3	TGGCCAAGCTGCTAAAGTAG	ACTGATCACAAGCCAAAGCA	50.0	45.0	57.9	58.0	1512-1556 bp	1.0	81.4
psaC-ndhE_p4	GCGCTCGGGAGAAGAATAAT	ACTGATCACAAGCCAAAGCA	50.0	45.0	57.8	58.0	1159-1203 bp	1.0	81.4
psaC-ndhE_p5	ATCGATTCCCAGTCTCCAGT	ACTGATCACAAGCCAAAGCA	50.0	45.0	57.8	58.0	1443-1487 bp	1.0	81.0