Hotspot

clpP-psbB

LSC · 73480-74299 · 820 bp

Back to Oreorchis

Pi 0.0175

MarkerSeek score 57.60

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 820
variable_sites: 21
pi: 0.0175
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	ACTCTTGGAACACCAATGGG	GGCCCAACCAGAAACTAGAG	50.0	55.0	57.7	57.9	945-997 bp	1.0	78.6
clpP-psbB_p2	ACTCTTGGAACACCAATGGG	GCCCAACCAGAAACTAGAGC	50.0	55.0	57.7	58.5	944-996 bp	1.0	75.2
clpP-psbB_p3	CTTGGAACACCAATGGGCAT	GGCCCAACCAGAAACTAGAG	50.0	55.0	58.7	57.9	942-994 bp	1.0	75.1
clpP-psbB_p4	ACTCTTGGAACACCAATGGG	ACAGAAAGTAATCGACCGGG	50.0	50.0	57.7	57.3	908-960 bp	1.0	74.3
clpP-psbB_p5	TCTTGGAACACCAATGGGC	GGCCCAACCAGAAACTAGAG	52.6	55.0	57.9	57.9	943-995 bp	1.0	72.6