Hotspot

psaJ-rpl33

LSC · 68407-69010 · 604 bp

Back to Oreorchis

Pi 0.0188

MarkerSeek score 60.50

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 604
variable_sites: 17
pi: 0.0188
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GATGCCTTGTCATTCCCCTT	CGAACACAACAGGTACATTCC	50.0	47.6	57.9	57.2	696-710 bp	1.0	66.3
psaJ-rpl33_p2	GATGCCTTGTCATTCCCCTT	CCTTTCGAACACAACAGGTAC	50.0	47.6	57.9	57.1	701-715 bp	1.0	65.7
psaJ-rpl33_p3	TGGTTTGGGTCTTTAGCAGG	CGAACACAACAGGTACATTCC	50.0	47.6	57.7	57.2	747-761 bp	1.0	65.1
psaJ-rpl33_p4	GGTTTGGGTCTTTAGCAGGT	CGAACACAACAGGTACATTCC	50.0	47.6	57.7	57.2	746-760 bp	1.0	65.1
psaJ-rpl33_p5	TGGTTTGGGTCTTTAGCAGG	CCTTTCGAACACAACAGGTAC	50.0	47.6	57.7	57.1	752-766 bp	1.0	64.5