Hotspot

ndhC-trnV-UAC

LSC · 51546-52236 · 691 bp

Back to Oreorchis

Pi 0.0145

MarkerSeek score 64.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 691
variable_sites: 15
pi: 0.0145
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	ACCAAGATAGGAATAACGCTTGA	AGAAGGTCTACGGTTCGAGT	39.1	50.0	57.6	57.8	757-795 bp	1.0	57.1
ndhC-trnV-UAC_p2	ACCAAGATAGGAATAACGCTTGA	AGCCCAATGCAACAAAATGC	39.1	45.0	57.6	58.5	839-877 bp	1.0	54.8
ndhC-trnV-UAC_p3	ACCAAGATAGGAATAACGCTTGA	GAAGGTCTACGGTTCGAGTC	39.1	55.0	57.6	57.5	756-794 bp	1.0	54.4
ndhC-trnV-UAC_p4	ACCAAGATAGGAATAACGCTTG	AGAAGGTCTACGGTTCGAGT	40.9	50.0	56.2	57.8	757-795 bp	1.0	53.9
ndhC-trnV-UAC_p5	ACCAAGATAGGAATAACGCTTGA	GTTCGAGTCCGTATAGCCCT	39.1	55.0	57.6	58.7	745-783 bp	1.0	53.3