Hotspot

psaA-ycf3

LSC · 42640-43291 · 652 bp

Back to Oreorchis

Pi 0.0153

MarkerSeek score 69.10

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 652
variable_sites: 15
pi: 0.0153
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCCATTCCTCGAAAGACGTT	ATAGCGCTTACTCCAGGGAA	50.0	50.0	57.9	58.2	798-800 bp	1.0	79.4
psaA-ycf3_p2	CCCATTCCTCGAAAGACGTT	TAGCGCTTACTCCAGGGAAT	50.0	50.0	57.9	58.2	797-799 bp	1.0	79.4
psaA-ycf3_p3	CCCATTCCTCGAAAGACGTT	GTTCGATCAAGCTGCTGAGT	50.0	50.0	57.9	58.3	832-834 bp	1.0	78.7
psaA-ycf3_p4	CCCATTCCTCGAAAGACGTT	GCTATAGCGCTTACTCCAGG	50.0	55.0	57.9	57.6	801-803 bp	1.0	78.2
psaA-ycf3_p5	CCCATTCCTCGAAAGACGTT	CTTGGTTCGATCAAGCTGCT	50.0	50.0	57.9	58.6	836-838 bp	1.0	76.5