Hotspot

rps16-trnQ-UUG

LSC · 6056-7042 · 987 bp

Back to Oreorchis

Pi 0.0182

MarkerSeek score 70.80

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 987
variable_sites: 26
pi: 0.0182
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	ATCCACAGCCAATCATGTCC	GACCAAATCAAAATGGGGCG	50.0	50.0	57.9	58.0	1087-1144 bp	1.0	85.3
rps16-trnQ-UUG_p2	ATCCACAGCCAATCATGTCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	1026-1083 bp	1.0	84.8
rps16-trnQ-UUG_p3	ATCCACAGCCAATCATGTCC	CAAGTGGTAAGGCAATGGGT	50.0	50.0	57.9	58.1	1063-1120 bp	1.0	84.8
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	GACCAAATCAAAATGGGGCG	45.0	50.0	58.1	58.0	1064-1121 bp	1.0	84.7
rps16-trnQ-UUG_p5	CCACAGCCAATCATGTCCTT	GACCAAATCAAAATGGGGCG	50.0	50.0	58.2	58.0	1085-1142 bp	1.0	84.5