Hotspot

rps16

LSC · 5148-6345 · 1198 bp

Back to Oreocnide

Pi 0.00197

MarkerSeek score 54.00

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1198
variable_sites: 10
pi: 0.00197
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	CAATGGGCGTTTTTCAGTGG	CCGACCGAACCAATGACTAT	50.0	50.0	58.2	57.4	1368-1396 bp	1.0	75.3
rps16_p2	CAATGGGCGTTTTTCAGTGG	GTACCGACCGAACCAATGAC	50.0	55.0	58.2	58.6	1371-1399 bp	1.0	74.9
rps16_p3	AATGGGCGTTTTTCAGTGGA	CCGACCGAACCAATGACTAT	45.0	50.0	58.3	57.4	1367-1395 bp	1.0	74.6
rps16_p4	ATGGGCGTTTTTCAGTGGAA	CCGACCGAACCAATGACTAT	45.0	50.0	58.3	57.4	1366-1394 bp	1.0	74.6
rps16_p5	TGGGCGTTTTTCAGTGGAAT	CCGACCGAACCAATGACTAT	45.0	50.0	58.3	57.4	1365-1393 bp	1.0	74.6