Hotspot

clpP

LSC · 72140-74245 · 2106 bp

Back to Oreocnide

Pi 0.0027

MarkerSeek score 43.40

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2106
variable_sites: 26
pi: 0.0027
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	ATTGGCTGTCGTGTGTTTCT	CGTATAGAGCCATCGAACCG	45.0	55.0	58.0	58.0	2863-2924 bp	1.0	82.4
clpP_p2	ATTGGCTGTCGTGTGTTTCT	ATACCAAGGCAAACCCATGG	45.0	50.0	58.0	58.1	2760-2821 bp	1.0	81.7
clpP_p3	ATTGGCTGTCGTGTGTTTCT	GCCGGGATCATTCAATACGA	45.0	50.0	58.0	57.8	2793-2854 bp	1.0	81.5
clpP_p4	ATTGGCTGTCGTGTGTTTCT	CGATACCAAGGCAAACCCAT	45.0	50.0	58.0	58.2	2762-2823 bp	1.0	80.9
clpP_p5	AGTCGCACATACACCCTAGT	CGTATAGAGCCATCGAACCG	50.0	55.0	58.2	58.0	2941-2991 bp	0.916667	77.9