Hotspot

ccsA-ndhD

SSC · 117953-118237 · 285 bp

Back to Oreocnide

Pi 0.0302

MarkerSeek score 64.80

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 285
variable_sites: 20
pi: 0.0302
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CCTCGACGGGCTTTCTTTTA	TCAGTTGACAAAGTCGAAGCT	50.0	42.9	57.9	57.8	434-443 bp	1.0	74.8
ccsA-ndhD_p2	CCTCGACGGGCTTTCTTTTA	AGTTGACAAAGTCGAAGCTCT	50.0	42.9	57.9	57.5	432-441 bp	1.0	72.6
ccsA-ndhD_p3	CCTCGACGGGCTTTCTTTTA	CAGTTGACAAAGTCGAAGCTC	50.0	47.6	57.9	57.5	433-442 bp	1.0	72.2
ccsA-ndhD_p4	CCTCGACGGGCTTTCTTTTA	TCAGTTGACAAAGTCGAAGC	50.0	45.0	57.9	56.3	434-443 bp	1.0	70.8
ccsA-ndhD_p5	CCTCGACGGGCTTTCTTTTA	CAGTTGACAAAGTCGAAGCT	50.0	45.0	57.9	56.3	433-442 bp	1.0	70.7