Hotspot

ndhF-rpl32

SSC · 114548-115810 · 1263 bp

Back to Oreocnide

Pi 0.00426

MarkerSeek score 61.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1263
variable_sites: 25
pi: 0.00426
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGCGAAGATCTTTTGTTGCC	AAAAGGCTTTCAATGCTGCC	50.0	45.0	58.0	57.8	1422-1487 bp	1.0	83.7
ndhF-rpl32_p2	CCACATACGGCGAAGATCTT	AAAAGGCTTTCAATGCTGCC	50.0	45.0	57.8	57.8	1430-1495 bp	1.0	82.0
ndhF-rpl32_p3	GCGAAGATCTTTTGTTGCCG	AAAAGGCTTTCAATGCTGCC	50.0	45.0	58.4	57.8	1421-1486 bp	1.0	80.7
ndhF-rpl32_p4	CGGCGAAGATCTTTTGTTGC	AAAAGGCTTTCAATGCTGCC	50.0	45.0	58.4	57.8	1423-1488 bp	1.0	80.6
ndhF-rpl32_p5	GGCGAAGATCTTTTGTTGCC	GCTTTCAATGCTGCCCAATA	50.0	45.0	58.0	57.0	1417-1482 bp	1.0	77.3