Hotspot

rpl16-rps3

LSC · 85009-85170 · 162 bp

Back to Oreocnide

Pi 0.00669

MarkerSeek score 51.20

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 162
variable_sites: 5
pi: 0.00669
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	ATGCGAAACGATGAGTTGGT	GGGAAGGTAGAGTGCCTCTA	45.0	55.0	58.2	57.5	371-380 bp	1.0	77.0
rpl16-rps3_p2	ATGCGAAACGATGAGTTGGT	ATCAGGGAAGGTAGAGTGCC	45.0	55.0	58.2	58.5	375-384 bp	1.0	76.6
rpl16-rps3_p3	TGCGAAACGATGAGTTGGTT	GGGAAGGTAGAGTGCCTCTA	45.0	55.0	58.4	57.5	370-379 bp	1.0	75.2
rpl16-rps3_p4	TGCGAAACGATGAGTTGGTT	ATCAGGGAAGGTAGAGTGCC	45.0	55.0	58.4	58.5	374-383 bp	1.0	74.8
rpl16-rps3_p5	ATGCGAAACGATGAGTTGGT	CAGGGAAGGTAGAGTGCCT	45.0	57.9	58.2	58.0	373-382 bp	1.0	72.6