Hotspot

psaJ-rpl33

LSC · 69194-69650 · 457 bp

Back to Oreocnide

Pi 0.00288
MarkerSeek score 51.80
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
457
variable_sites
7
pi
0.00288
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 ACATATCTTTCCGTCGCACC ACTCGAGCTTCTTTACCCCT 50.0 50.0 58.1 58.1 591-611 bp 1.0 81.8
psaJ-rpl33_p2 TCGCACCGGTAGTAAGTACT ACTCGAGCTTCTTTACCCCT 50.0 50.0 57.9 58.1 578-598 bp 1.0 81.5
psaJ-rpl33_p3 GTCGCACCGGTAGTAAGTAC ACTCGAGCTTCTTTACCCCT 55.0 50.0 57.8 58.1 579-599 bp 1.0 80.9
psaJ-rpl33_p4 ACATATCTTTCCGTCGCACC CTCGAGCTTCTTTACCCCTG 50.0 55.0 58.1 57.7 590-610 bp 1.0 79.9
psaJ-rpl33_p5 TCGCACCGGTAGTAAGTACT CTCGAGCTTCTTTACCCCTG 50.0 55.0 57.9 57.7 577-597 bp 1.0 79.5