Hotspot

rps4-trnT-UGU

LSC · 47223-47723 · 501 bp

Back to Oreocnide

Pi 0.0016

MarkerSeek score 53.20

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 501
variable_sites: 4
pi: 0.0016
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	CGGGGCGTAATTGGTTTCTA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.0	57.9	624-685 bp	1.0	83.4
rps4-trnT-UGU_p2	CGTTTCCGGCTATAGGCTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.1	57.9	600-661 bp	1.0	83.3
rps4-trnT-UGU_p3	CGGGGCGTAATTGGTTTCTA	GATGGTCATCGGTTCGACTC	50.0	55.0	58.0	58.2	593-654 bp	1.0	83.3
rps4-trnT-UGU_p4	CGTTTCCGGCTATAGGCTTT	GATGGTCATCGGTTCGACTC	50.0	55.0	58.1	58.2	569-630 bp	1.0	83.1
rps4-trnT-UGU_p5	CCCCCAGACGACGTATTTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.9	57.9	558-619 bp	1.0	82.8