Hotspot

trnC-GCA-petN

LSC · 28373-29366 · 994 bp

Back to Oreocnide

Pi 0.0032

MarkerSeek score 57.60

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 994
variable_sites: 12
pi: 0.0032
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTTGTATCGTTTTGGCGGC	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.9	58.2	1054-1123 bp	1.0	83.3
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	998-1067 bp	1.0	83.1
trnC-GCA-petN_p3	GGGTGTCGCCTGATTAACAA	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.2	58.2	980-1049 bp	1.0	82.3
trnC-GCA-petN_p4	TTTTGTATCGTTTTGGCGGC	AGAGTCCACTTCTTCCCCAT	45.0	50.0	57.9	57.6	1115-1184 bp	1.0	81.7
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	1059-1128 bp	1.0	81.6