Hotspot

trnK-UUU-rps16

LSC · 4336-5147 · 812 bp

Back to Oreocnide

Pi 0.00323
MarkerSeek score 54.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
812
variable_sites
13
pi
0.00323
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGTCGCGGTCTTACAAACTC ATTTCAAAGAAGGCGGGTGT 50.0 45.0 57.9 58.0 976-995 bp 1.0 82.3
trnK-UUU-rps16_p2 GTCGCGGTCTTACAAACTCT ATTTCAAAGAAGGCGGGTGT 50.0 45.0 57.9 58.0 975-994 bp 1.0 82.3
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT ATTTCAAAGAAGGCGGGTGT 50.0 45.0 58.2 58.0 893-912 bp 1.0 81.6
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT ATTTCAAAGAAGGCGGGTGT 50.0 45.0 58.2 58.0 892-911 bp 1.0 81.6
trnK-UUU-rps16_p5 CAGTCGCGGTCTTACAAACT ATTTCAAAGAAGGCGGGTGT 50.0 45.0 58.2 58.0 977-996 bp 1.0 81.0