Hotspot

ycf1

SSC · 123408-128873 · 5466 bp

Back to Oreocharis

Pi 0.0154

MarkerSeek score 48.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5466
variable_sites: 256
pi: 0.0154
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCGTTCCATTGACTCGGATT	GGAAGAAGGGGTGGAAAGTG	50.0	55.0	58.0	58.1	155-179 bp	0.875	85.9
ycf1_p2	CCGTTCCATTGACTCGGATT	AGGGCCCTCTTATCTCTTCC	50.0	55.0	58.0	57.9	653-824 bp	1.0	85.0
ycf1_p3	CCGTTCCATTGACTCGGATT	GGGCCCTCTTATCTCTTCCT	50.0	55.0	58.0	57.9	652-823 bp	1.0	85.0
ycf1_p4	CCGTTCCATTGACTCGGATT	TCCGAGCTCAGGTTATGGAA	50.0	50.0	58.0	58.1	630-801 bp	1.0	84.9
ycf1_p5	CCGTTCCATTGACTCGGATT	GCAGAATACCGTCACCCATT	50.0	50.0	58.0	58.0	207-231 bp	0.875	84.5