Hotspot

ndhF

SSC · 110212-112497 · 2286 bp

Back to Oreocharis

Pi 0.0128

MarkerSeek score 47.00

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2286
variable_sites: 91
pi: 0.0128
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CGATGCAACAACAAGACGTT	ACGATGCACAATACCCCTTC	45.0	50.0	57.6	58.0	2930 bp	0.125	41.6
ndhF_p2	TCGATGCAACAACAAGACGT	ACGATGCACAATACCCCTTC	45.0	50.0	58.4	58.0	2931 bp	0.125	41.3
ndhF_p3	TTCGATGCAACAACAAGACG	ACGATGCACAATACCCCTTC	45.0	50.0	57.3	58.0	2932 bp	0.125	39.4
ndhF_p4	CGATGCAACAACAAGACGTT	AACGATGCACAATACCCCTT	45.0	45.0	57.6	57.5	2931 bp	0.125	37.9
ndhF_p5	TCGATGCAACAACAAGACGT	AACGATGCACAATACCCCTT	45.0	45.0	58.4	57.5	2932 bp	0.125	37.6