Hotspot

atpA

LSC · 10499-12022 · 1524 bp

Back to Oreocharis

Pi 0.003

MarkerSeek score 40.00

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 13
pi: 0.003
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	AGCCTTCCAAGCTAACGATG	TGGAAATGGAAGCCGATGAG	50.0	50.0	58.0	58.0	2143-2260 bp	1.0	85.5
atpA_p2	AGCCTTCCAAGCTAACGATG	GGAAATGGAAGCCGATGAGT	50.0	50.0	58.0	58.0	2142-2259 bp	1.0	85.5
atpA_p3	AGCCTTCCAAGCTAACGATG	GGAAGCCGATGAGTTTAGGG	50.0	55.0	58.0	58.0	2136-2253 bp	1.0	85.5
atpA_p4	AGCCTTCCAAGCTAACGATG	GTAATTGAGCGTGAGAGCCA	50.0	50.0	58.0	58.0	2360-2477 bp	1.0	85.4
atpA_p5	CCTAGCCTTCCAAGCTAACG	GTAATTGAGCGTGAGAGCCA	55.0	50.0	58.1	58.0	2363-2480 bp	1.0	85.1