Hotspot

rps15-ycf1

SSC · 123042-123407 · 366 bp

Back to Oreocharis

Pi 0.0191

MarkerSeek score 49.30

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 366
variable_sites: 21
pi: 0.0191
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCCCAACGCGTTACATAAGG	AGACCCCATTCATTTGCCTG	50.0	50.0	58.0	58.1	1475-1497 bp	1.0	82.1
rps15-ycf1_p2	AACACCATGCATTGATGGGT	AGACCCCATTCATTTGCCTG	45.0	50.0	58.1	58.1	1338-1360 bp	1.0	82.0
rps15-ycf1_p3	TCCCAACGCGTTACATAAGG	ATTTGCCTGGAAACGACGAT	50.0	45.0	58.0	58.2	1464-1486 bp	1.0	81.9
rps15-ycf1_p4	CCGAGCCATAACAAATGGGA	AGACCCCATTCATTTGCCTG	50.0	50.0	57.9	58.1	1619-1641 bp	1.0	81.8
rps15-ycf1_p5	TCCCAACGCGTTACATAAGG	CTGGAAACGACGATGGACAA	50.0	50.0	58.0	58.2	1458-1477 bp	0.5	60.6