Hotspot

ndhD-psaC

SSC · 116767-116885 · 119 bp

Back to Oreocharis

Pi 0.0549

MarkerSeek score 69.70

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 119
variable_sites: 16
pi: 0.0549
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	ACCTGCTGATATTGGCAAAAC	GCCTGTCCAACCGATTTCTT	42.9	50.0	57.1	58.5	251 bp	1.0	66.4
ndhD-psaC_p2	GAACCTGCTGATATTGGCAA	GCCTGTCCAACCGATTTCTT	45.0	50.0	56.1	58.5	253 bp	1.0	66.0
ndhD-psaC_p3	GAACCTGCTGATATTGGCAAA	GCCTGTCCAACCGATTTCTT	42.9	50.0	56.9	58.5	253 bp	1.0	64.1
ndhD-psaC_p4	CCTGCTGATATTGGCAAAACT	GCCTGTCCAACCGATTTCTT	42.9	50.0	56.8	58.5	250 bp	1.0	64.1
ndhD-psaC_p5	AACCTGCTGATATTGGCAAAAC	GCCTGTCCAACCGATTTCTT	40.9	50.0	57.8	58.5	252 bp	1.0	63.8