Hotspot

rps19-rpl2

IRb · 84933-84987 · 55 bp

Back to Oreocharis

Pi 0.0273

MarkerSeek score 56.30

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 55
variable_sites: 6
pi: 0.0273
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	TGCTGCTGCAGAATAAACCA	AAAAGTTTGGGTAGAGCCGG	45.0	50.0	58.1	58.1	802-804 bp	1.0	80.2
rps19-rpl2_p2	TGCTGCTGCAGAATAAACCA	ATTACCTTCTGGGGAGGTCC	45.0	55.0	58.1	58.1	884-886 bp	1.0	80.0
rps19-rpl2_p3	TGCTGCTGCAGAATAAACCA	TGGGGAGGTCCGTTTGATAT	45.0	50.0	58.1	58.1	875-877 bp	1.0	79.9
rps19-rpl2_p4	TGCTGCTGCAGAATAAACCA	GCGAACCAGAAAAGTTTGGG	45.0	50.0	58.1	57.9	811-813 bp	1.0	79.9
rps19-rpl2_p5	TGCTGCTGCAGAATAAACCA	GGCGAACCAGAAAAGTTTGG	45.0	50.0	58.1	57.9	812-814 bp	1.0	79.9