Hotspot

psbT-pbf1

LSC · 75318-75382 · 65 bp

Back to Oreocharis

Pi 0.0582

MarkerSeek score 68.40

Primer available yes

Label psbT-pbf1

Feature Metadata

label_name: psbT-pbf1
feature_type: igs
region: LSC
length_bp: 65
variable_sites: 9
pi: 0.0582
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-pbf1_p1	CGAGAACCACCTAAAGTTCCA	TCGAGGAACATGGGGACTAA	47.6	50.0	57.6	57.8	107-118 bp	1.0	77.2
psbT-pbf1_p2	CGAGAACCACCTAAAGTTCCA	TTCGAGGAACATGGGGACTA	47.6	50.0	57.6	57.8	108-119 bp	1.0	77.2
psbT-pbf1_p3	CGAGAACCACCTAAAGTTCCA	CATTCGAGGAACATGGGGAC	47.6	55.0	57.6	58.3	110-121 bp	1.0	76.3
psbT-pbf1_p4	CGAGAACCACCTAAAGTTCCA	ATTCGAGGAACATGGGGACT	47.6	50.0	57.6	58.4	109-120 bp	1.0	75.7
psbT-pbf1_p5	TCGAGAACCACCTAAAGTTCC	TCGAGGAACATGGGGACTAA	47.6	50.0	57.3	57.8	108-119 bp	1.0	75.0