Hotspot

trnF-GAA-ndhJ

LSC · 49076-49739 · 664 bp

Back to Oreocharis

Pi 0.0144

MarkerSeek score 55.30

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 664
variable_sites: 30
pi: 0.0144
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAATACAAGATGCTCATTGA	50.0	31.8	58.1	53.8	716-731 bp	1.0	46.0
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	TGAAATACAAGATGCTCATTGA	55.0	31.8	58.0	53.8	752-767 bp	1.0	45.7
trnF-GAA-ndhJ_p3	CTACATTGGGAATGGTCGGG	TGAAATACAAGATGCTCATTGA	55.0	31.8	58.0	53.8	769-784 bp	1.0	45.6
trnF-GAA-ndhJ_p4	TGCTACATTGGGAATGGTCG	TGAAATACAAGATGCTCATTGA	50.0	31.8	58.0	53.8	771-786 bp	1.0	45.6
trnF-GAA-ndhJ_p5	GGATGGGATGCTACATTGGG	TGAAATACAAGATGCTCATTGA	55.0	31.8	58.1	53.8	779-794 bp	1.0	45.5