Hotspot

atpF-atpH

LSC · 13343-13726 · 384 bp

Back to Oreocharis

Pi 0.00614

MarkerSeek score 56.30

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 384
variable_sites: 7
pi: 0.00614
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CCAGTGGCCTAAAGAAACGA	AGGCAGAGGGAAAGATACGA	50.0	50.0	57.8	57.8	526-544 bp	1.0	81.0
atpF-atpH_p2	TTAAATCCGAAACTCCCGGC	AGGCAGAGGGAAAGATACGA	50.0	50.0	58.3	57.8	552-570 bp	1.0	80.2
atpF-atpH_p3	CCAGTGGCCTAAAGAAACGA	GAGGCAGAGGGAAAGATACG	50.0	55.0	57.8	57.5	527-545 bp	1.0	78.1
atpF-atpH_p4	CCAGTGGCCTAAAGAAACGA	GCAGAGGGAAAGATACGAGG	50.0	55.0	57.8	57.5	524-542 bp	1.0	78.1
atpF-atpH_p5	CCAGTGGCCTAAAGAAACGA	GGCAGAGGGAAAGATACGAG	50.0	55.0	57.8	57.5	525-543 bp	1.0	78.1