Hotspot

rps15

SSC · 121281-121553 · 273 bp

Back to Orchis

Pi 0
MarkerSeek score 32.40
Primer available yes
Label rps15

Feature Metadata

label_name
rps15
feature_type
gene
region
SSC
length_bp
273
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
32.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15_p1 TGCTAATAACTGAACTAAGAAAAA TCCTGATCAGTAAAATTCACAGA 25.0 34.8 52.7 55.3 410 bp 1.0 40.0
rps15_p2 TCTAATGCTAATAACTGAACTAAGA TCCTGATCAGTAAAATTCACAGA 28.0 34.8 53.6 55.3 415 bp 1.0 40.0
rps15_p3 AATGCTAATAACTGAACTAAGAAA TCCTGATCAGTAAAATTCACAGA 25.0 34.8 52.5 55.3 412 bp 1.0 40.0
rps15_p4 ATGCTAATAACTGAACTAAGAAAA TCCTGATCAGTAAAATTCACAGA 25.0 34.8 52.5 55.3 411 bp 1.0 40.0
rps15_p5 TGCTAATAACTGAACTAAGAAAAA TTCCTGATCAGTAAAATTCACAGA 25.0 33.3 52.7 56.1 411 bp 1.0 40.0