Hotspot

rpoC1

LSC · 19613-22403 · 2791 bp

Pi 0.000358

MarkerSeek score 56.30

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2791
variable_sites: 1
pi: 0.000358
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	ACACGAGTATGGGCATCACT	TTGCTCGTTCGAGAACTACG	50.0	50.0	58.8	58.0	2971 bp	1.0	73.3
rpoC1_p2	TACACGAGTATGGGCATCAC	TTGCTCGTTCGAGAACTACG	50.0	50.0	57.1	58.0	2972 bp	1.0	72.6
rpoC1_p3	TTACACGAGTATGGGCATCAC	TTGCTCGTTCGAGAACTACG	47.6	50.0	57.8	58.0	2973 bp	1.0	70.3
rpoC1_p4	CACGAGTATGGGCATCACTA	TTGCTCGTTCGAGAACTACG	50.0	50.0	56.8	58.0	2970 bp	1.0	70.3
rpoC1_p5	ACACGAGTATGGGCATCACT	GCTCGTTCGAGAACTACGAT	50.0	50.0	58.8	57.5	2969 bp	1.0	69.9